MoBio Production of Recombinant Proteins Chapter 9

Many proteins (such as insulin) which may be used for medical treatment or for research are normally expressed at very low concentrations. Through recombinant DNA technology, a large quantity of proteins can be produced. This involves inserting the desired protein gene into an "expression vector" which must contain a promoter so that the protein can be expressed.

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Figure 9-H-1. Production of recombinant proteins. (a) The expression vector contains the lac promoter and its neighboring lacZ gene encoding β-galactosidase. Lactose or its analog IPTG can stimulate the expression of β-galactosidase. (b) If lacZ is replaced by the gene encoding the protein of interest, lactose or IPTG will stimulate the expression of desired proteins.