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Many proteins which may be used for medical treatment or for research are normally expressed at very low concentrations. Through recombinant DNA technology, a large quantity of proteins can be produced. This involves inserting the desired protein gene into an "expression vector" which must contain a promoter so that the protein can be expressed.
Figure 9-E-3. Production of recombinant proteins. (a) The expression vector contains the lac promoter and its neighboring lacZ gene encoding b-galactosidase. Lactose or its analog IPTG will stimulate the expression of b-galactosidase. (b) If lacZ is replaced by the gene encoding the protein of interest, lactose or IPTG will stimulate the expression of desired proteins.
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