|MoBio||Protein Microarray||Chapter 9|
Protein microarray, also known as protein chip, is a method to study protein activities on a large scale. It has been applied to proteomics. While the word "genomics" refers to the study of a genome, proteomics is the study of a proteome - the entire set of expressed proteins in a cell at a given time. Compared to the old techniques, gel electrophoresis and chromatography, protein microarray is much more efficient in proteomics.
Protein microarray has two basic formats: forward phase and reverse phase.
Forward phase protein microarray
Antibodies or other proteins are arrayed at defined positions on a glass slide or other support surface. The array is then probed with a variety of molecules, such as protein lysate, enzymes, small molecules and nucleic acids. Analysis of the resulting binding reactions can provide information about expression levels of particular proteins in the sample as well as binding affinities and specificities.
Rreverse phase protein microarray
Protein lysate is arrayed onto the microarray slide and probed with antibodies against the target protein of interest.