MoBio Restriction Enzymes Chapter 9
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Table 9-A-1. Commonly used restriction enzymes. The "Recognition site" of a restriction enzyme is also called the restriction site. In this column, the first line is from 5' to 3' and the second line is from 3' to 5'. The arrow indicates the cleavage site. If the cleavage site is not at the center, the restriction enzyme will generate sticky ends which can base-pair with other DNA fragments cleaved by the same restriction enzyme. If the cleavage site is at the center, the restriction enzyme will generate blunt ends.