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The procedure used in artificial DNA recombination is similar to the natural transpositional recombination. The major difference is that researchers can choose any appropriate enzymes to cut the DNA molecules. They are usually isolated from bacteria. The role of these enzymes in bacteria is to "restrict" the invasion of foreign DNA by cutting it into pieces. Hence, these enzymes are known as restriction enzymes. Table 9-A-1. Commonly used restriction enzymes.
Note: The "Recognition site" of a restriction enzyme is also called the restriction site. In this column, the first line is from 5' to 3' and the second line is from 3' to 5'. The arrow indicates the cleavage site. If the cleavage site is not at the center, the restriction enzyme will generate sticky ends (web link) which can base-pair with other DNA fragments cleaved by the same restriction enzyme. If the cleavage site is at the center, the restriction enzyme will generate blunt ends.
Other sites of Interest:
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