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Genomes and Proteomics

 


Genomes

"Genome"is the total genetic information of an organism. For most organisms, it is the complete DNA sequence. For RNA viruses, the genome is the complete RNA sequence, since their genetic information is encoded in RNA.

Table 3-H-1.  The genomes of prominent organisms. 

Organism Genome Size (Mb) Gene Number
Hepatitis D virus 0.0017 2
Hepatitis B virus 0.0032 4
HIV-1 0.0092 9
Bacteriophage l 0.0485 90
Escherichia coli 4.6 4437
S. cerevisiae (yeast) 12 6300
C. elegans (nematode) 97 19000
D. melanogaster (fruit fly) 137 14000
Mus musculus (mouse) 3000 20000-30000
Homo sapiens (human) 3000 20000-30000

* 1 Mb = 1 million base pairs (for double-stranded DNA or RNA) or 1 million bases (for single-stranded DNA or RNA).

 

The Genomes of Mitochondria and Chloroplasts


Sites of Interest:

Review Articles:

Form follows function: the genomic organization of cellular differentiation - Genes and Develop., 2004.

Lessons from the Genome Sequence of Neurospora crassa - Microbiol. and Mol. Biol. Rev., 2004.

Nutritional genomics: the next frontier in the postgenomic era - Physiol. Genomics, 2004.

Structural genomics: Computational methods for structure analysis - Protein Science, 2003.

The sense of smell: genomics of vertebrate odorant receptors - Hum. Mol. Genet., 2002.

 

Proteomics

"Proteomics" is the study of  "proteome", which contains all proteins in a cell at a particular time. While all cells in an organism have the same genome, they usually have different proteomes.

Sites of Interest:

ExPASy Proteomics tools

 

Review Articles:

Interactome: gateway into systems biology

Series from Mole. Cell. Proteomics, 2005

Proteomics techniques and their application to hematology - Blood, 2004.

Chemical Strategies for Functional Proteomics - Mol. Cellu. Proteomics, 2002.

Proteomics for Cancer Biomarker Discovery - Clin. Chem., 2002.

Applied Proteomics - Circulation Research, 2002.

Proteomic Analysis in the Neurosciences - Mol. Cellu. Proteomics, 2002.

Charting the Protein Complexome in Yeast by Mass Spectrometry - Mol. Cellu. Proteomics, 2002.

Cardiovascular Proteomics - Circulation Research, 2001.

Advances in Proteome Analysis by Mass Spectrometry - J. Biol. Chem., 2001.