|MoBio||Cracking the Genetic Code||Chapter 3|
Individual trinucleotide and aminoacyl-tRNA can pass through the filter, but the ribosome is too big to pass through. Therefore, if the labeled aminoacyl-tRNA contains the anticodon for the trinucleotide, it will bind to the trinucleotide and ribosome on the filter. In this case, the radioactivity can be detected on the filter and the amino acid in the labeled aminoacyl-tRNA is likely to be encoded by the trinucleotide. If no radioactivity was detected, the trinucleotide is unlikely to be the codon of the amino acid. Most of the 64 possible codons can be determined by repeating this procedure for different trinucleotides and labellings.